家电科技 ›› 2020, Vol. 0 ›› Issue (2): 41-43.doi: 10.19784/j.cnki.issn1672-0172.2020.02.004

• 论文 • 上一篇    下一篇

从脲酶活性探讨不同海拔高度下豆浆机的健康安全

孟晓山, 张晓   

  1. 中国家用电器研究院 北京 100053
  • 出版日期:2020-04-01 发布日期:2020-03-31
  • 作者简介:孟晓山,mengxsh@cheari.com

Investigate the health security of soymilk maker at different altitudes from the urease activity

MENG Xiaoshan, ZHANG Xiao   

  1. China Household Electric Appliance Research Institute Beijing 100053
  • Online:2020-04-01 Published:2020-03-31

摘要: 为探讨不同海拔高度对豆浆机煮熟度性能的影响,研究测试了3款主流品牌的豆浆机在制备豆浆过程中脲酶活性的变化,考查了不同海拔1500m、3000m和4500m水沸点下豆浆中脲酶活性的变化规律,并探讨了采用分光光度计验证上述判定结果的可行性。研究发现,3款主流品牌豆浆机在海拔50m(39°53'N,116°21'E)下使用时所制豆浆中脲酶活性均达标。然而,海拔的上升、沸点的降低导致需要更长的时间才能将脲酶灭活,为设计高原专供豆浆机提供了依据。采用分光光度计在420nm工作波长下测定待测试样的吸光度,并以吸光度作为评判脲酶是否失活的依据,避免个体色觉差异造成误判,为推动相关标准方法的修订和完善提供了依据。

关键词: 豆浆机, 脲酶, 沸点, 海拔, 分光光度法

Abstract: :To investigate the effects of altitude on the performance of soymilk maker, the variation of urease activity was tested during the boiling process by three leading brands of soymilk maker. The urease activity was also determined at different water boiling points corresponding to the altitudes of 1500m, 3000m and 4500m. Finally, the feasibility of using spectrophotometer to verify the results was discussed. The results indicated that three soymilk makers met the requirement of standard at the attitude of 50m (39°53'N, 116°21'E). However, more time was essential when the attitude increases and boiling point decreases, laying basis for designing soymilk maker exclusively used in plateau. Besides, employing spectrophotometry at 420nm to measure the absorbance of samples could avoid the optical disparity among individuals, providing the foundation for promoting the amendment and perfection of relative standard.

Key words: Soybean milk maker, Urease, Boiling point, Altitude, Spectrophotometry

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